Journal: Antimicrobial Agents and Chemotherapy

Article Title: Effect of Fluorescent Dyes on In Vitro -Differentiated, Late-Stage Plasmodium falciparum Gametocytes

doi: 10.1128/AAC.03772-14

Figure Lengend Snippet: In vitro activities of standard antimalarial drugs and dyes against stage V gametocytes of the P. falciparum 3D7 laboratory strain and clinical isolates after 48 h of incubation a

Article Snippet: Artesunate and primaquine were from Santa Cruz Biotechnology. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG 2 caption a7 Chemical structures of the tested synthetic dyes.

Techniques: In Vitro, Incubation

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Effect of Fluorescent Dyes on In Vitro -Differentiated, Late-Stage Plasmodium falciparum Gametocytes

doi: 10.1128/AAC.03772-14

Figure Lengend Snippet: In vitro activities of compounds against mature gametocytes of 3D7 and clinical isolates of P. falciparum after 24 h or 48 h of incubation a

Article Snippet: Artesunate and primaquine were from Santa Cruz Biotechnology. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG 2 caption a7 Chemical structures of the tested synthetic dyes.

Techniques: In Vitro, Incubation

Journal: The Journal of General Physiology

Article Title: Rapid resensitization of ASIC2a is conferred by three amino acid residues in the N terminus

doi: 10.1085/jgp.201812224

Figure Lengend Snippet: The prolonged desensitization of the ASIC2a mutants is not due to an internalization of the ion channels in response to extracellular protons. (A) Currents elicited by three consecutive applications of pH 4.0 for 20 s in HEK293T cells expressing either Mut2 (upper) or Mut3 (lower) in the absence (blue) or presence (red) of 50 µM primaquine, begun 1 h before the recordings. (B) Peak current densities in cells expressing either Mut2 (upper) or Mut3 (lower) were normalized to those evoked by the initial acidic stimulus in the absence (blue) or presence (red) of 50 µM primaquine. n = 5 for each group. Acidic stimuli were delivered by switching the bath solution from pH 7.4 to pH 4.0 at 2-min intervals. (C and D) Western blotting showing the amounts of GFP-tagged Mut2 and Mut3 in the plasma membrane (PM) and total cell lysate (Total) following application of pH 7.4 versus pH 4.0. E-cadherin and GAPDH are markers used to normalize the amounts of Mut2 and Mut3 in the plasma membrane and total cell lysate, respectively. n = 3 for each group. n.s. not significant; Mann–Whitney U test.

Article Snippet: Transfected HEK293 cells were pretreated for 1 h with primaquine (50 μM; LKT Laboratories), and the bath solution was continuously perfused with primaquine over the course of the recording.

Techniques: Expressing, Western Blot, Clinical Proteomics, Membrane, MANN-WHITNEY

Journal: Analytical and Bioanalytical Chemistry

Article Title: Rapid evaluation of artesunate quality with a specific monoclonal antibody-based lateral flow dipstick

doi: 10.1007/s00216-016-9363-9

Figure Lengend Snippet: Fig. 5 Dipstick specificity tests of the partner drugs in the artesunate- based combinations. Artesunate solution was diluted in distilled water at a concentration of 2000 ng mL−1, while the other standard solutions were diluted in distilled water at a concentration of 20,000 ng mL−1. (1) blank, (2) artesunate, (3) amodiaquine, (4) pyronaridine tetraphosphate, (5) piperaquine tetraphosphate tetrahydrate, (6) primaquine phosphate, (7) pyrimethamine, (8) quinine, (9) chloroquine diphosphate salt, and (10) lumefantrine

Article Snippet: Quinine and primaquine phosphate were purchased from J&K Chemical (Beijing, China).

Techniques: Concentration Assay